Key words: Listeria monocytogenes, poultry, processing plant, evisceration. ABSTRACT . Listeria antisera (Denka Seiken, Tokyo, Japan), accord- ing to the . Caracterização feno e genotípica de cepas de Listeria monocytogenes isoladas They were also serotyped (Denka Seiken, Japan), and sub–typed by PFGE. Antisera for the typing of Listeria Group O and H antigens. Aids public health and food testing laboratories in identifying sources of infection/contamination to.
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From January to Maythirteen isolates of L. The antimicrobial seikdn of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. None of the isolates showed resistance to the antibiotics tested; however, seven of them presented increased values for sulfamethoxazole MICs. After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. It was observed that the spatial or temporally unrelated strains exhibited similar PFGE profiles, indicating a possible clonal relationship among them.
Listeria monocytogenes ; serotypes; antibiotic resistance; genotypic characterization; listeriosis. Nenhuma das cepas foi resistente aos antimicrobianos testados; entretanto, sete tiveram valores da CIM aumentados para o sulfametoxazol.
It may, however, have more severe consequences for the infant, including spontaneous abortion, fetal death, stillbirth, severe neonatal septicemia, and meningitis. Gastroenteritis symptoms vomiting and diarrhea are observed in some cases, during the week previous to disease diagnosis 9, While very rarely mentioned in the literature until recently, few cases of listeriosis have been reported in previously healthy persons and such cases were attributed to exposure to high infective doses, as well as outbreaks of benign febrile gastroenteritis 2,8, Therefore, a more accurate study of such infection is necessary in our country.
In general, strains of L. The therapy of choice is a combination of ampicillin and an aminoglycoside, usually gentamicin. Traditional epidemiological surveillance alone may not detect many common source outbreaks, particularly if a limited number of cases occur over a wide geographic area, due to the unique characteristics of human foodborne listeriosis.
Molecular subtyping methods are tools that allow detecting outbreaks and identifying their common source. Considering the lack of information available in Brazil concerning listeriosis, the aim of this study was to analyze phenotypic and genotypically the L. From January to May13 isolates from clinical materials seven from cerebrospinal fluid, five from blood and one from a liquid collected from an aorta prosthesis of 12 patients 11 adults and one newborn were identified as L.
The strains were identified in accordance with Rocourt et al. All other antimicrobial agents tested had no standardized breakpoints for Listeria spp.
Fragment patterns were analyzed according to Tenover et al. No strain was resistant to ampicillin, gentamicin, trimethoprim and vancomycin Table 1. The analysis of macrorestriction patterns obtained by PFGE and the combination of the results obtained with both restriction enzymes, ApaI Fig. The thirteen isolates listeira to three serotypes: Most industrialized countries have developed surveillance systems for listeriosis since 25following outbreaks that demonstrated the importance of the foodborne transmission of the disease.
Listeriosis has been mainly reported by those countries, with few or listeriia no reports from Africa, Asia and South America. According to Rocourt et al. In order to help filling this gap, collecting some data from Brazil was one of the aims of this research.
In this study, all the strains were susceptible to the antibiotics tested, except for sulfamethoxazole. It is opportune to emphasize the importance of using a standardized method to test bacterial resistance to antibiotics.
The disk diffusion method, although widely used, is not standardized nor recommended for fastidious bacteria like Listeria spp. Thus, the Brazilian data on antibiotic resistance by L. The most active agent in this combination seems to be trimethoprim, which is synergized by sulfamethoxazole.
In this study, these two drugs were tested individually and none of the strains showed resistance to trimethoprim. Additionally, there were increased values for sulfamethoxazole MICs Table 1. Nevertheless, these data were of particular concern since emergence and dissemination of antibiotic resistance in clinical isolates of L. Group I can be clearly divided in two clusters A and B with all the strains from cluster A belonging to serotype 4b. Unfortunately, in the present listfria the sources of exposure were undetermined for all the 12 clinical cases evaluated and no epidemiological data were also available, for this reason no conclusive relationships could be determined.
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According to our results, the proportion of L. However, this current favorable situation of quasiuniform susceptibility to the antibiotics used in clinical practice may deteriorate under the selective pressure exerted by overuse of the drugs in the country. In Brazil, few clinical cases of listeriosis have lisetria reported and none of them had their source of infection established.
In addition, once listeriosis is not a disease under compulsory notification, there are no registers identifying L. Whether this reflects the absence of cases or a lack of testing facilities is unknown. Furthermore, even when an early diagnosis would be possible, the long time for the onset of the clinical symptoms difficult the identification of the source of infection.
Subtyping eeiken will only provide their full public health benefit if food histories were routinely listwria for all listeriosis patients to provide an epidemiological support for putative single genotype clusters.
Finally, it is sseiken that the cases documented here may not represent the true incidence in the community, especially with regard to perinatal infection, once many laboratories of the liseria covered by this study do not often send the isolates for further characterization by the Regional Lab.
Sometimes, such labs are not capacitated to make a correctly identification of this bacterium. To improve such scenario, public health laboratories in Brazil should become able to isolate and identify L. Only by developing such capabilities, the true incidence of human listeriosis in Brazil could be established.
An outbreak of febrile gastroenteritis associated with corn contaminated by Listeria monocytogenes. Incidence of antibiotic resistance in Listeria species. Antibiotic resistance in Listeria spp. An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk.
Virulence characteristics of Listeria monocytogenes. Listeria monocytogenes in renal transplant recipients. Relato de casos em pacientes do Distrito Federal. Species listrria serovars of the genus Listeria isolated from different sources in Brazil from to Surto de meningite neonatal por Listeria monocytogenes. Influence of environmental conditions on the expression of virulence factors by Listeria monocytogenes and their use in species identification.
National Committee for Clinical Laboratory Standards: Susceptibilidade aos antimicrobianos de Listeria spp. Characterization of brazilian Listeria monocytogenes strains using DNA macrorestriction patterns. Antimicrobial resistance of Listeria monocytogenes. Epidemiology of human listeri and seafoods. Molecular subtyping to detect human listeriosis clusters.
Serotyping of Listeria monocytogenes and related species.
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Academic Press, London, p. Listeria monocytogenes infection in Israel and review of cases worldwide. August 31, ; Returned to authors for corrections: January 11, ; Approved: All the contents of this journal, except where otherwise noted, is licensed under seiekn Creative Commons Attribution License.
Services on Demand Journal. Serotyping The thirteen isolates belonged to three serotypes: How to cite this article.